Author Archives: mostafa

Real-Time PCR Instruments

BIO-RAD CFX96™

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The six-channel system combines advanced optical technology with precise thermal control to deliver sensitive, reliable detection. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen. With up to five-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful yet easy-to-use software, the CFX96 Touch system is designed to advance your qPCR.

Key Features and Benefits

With the CFX96 Touch Real-time PCR detection system you can:

  • Set up your system quickly — easy installation and factory-calibrated optics
  • Minimize sample and reagent use — up to 5-target multiplexing with sample volumes as low as 10 µl
  • Optimize reactions in a single run — thermal gradient feature
  • Analyze data faster — visualize all run data at once and export only the data you need in the format you want
  • Use advanced data analysis tools — normalized gene expression using CFX Manager™ software
  • Configure the system to fit your needs — run without a computer, run up to 4 systems from 1 computer, or integrate with the CFX automation system for higher throughput

INCEPTRA 9660

With Inceptra, product of advanced technology, the user will find it easy to perform both routine diagnostics tests and research.

Key Features and Benefits

●   Inceptra® has a broad linear range. It can detect fluorescence up to 10 orders of start DNA copies without the need for gradient dilution.

●   Automatic Hot-lid technology ensures smooth polymerase chain reaction even in test tubes with different heights; and prevents evaporation of reagents by applying constant pressure.

●   The adopted fluorescent real-time detection mode realizes simultaneous amplification and detection in the same tube without any need for post- treatment. Consequently it is possible to monitor the reaction on-line and intervene when necessary.

●   As a light source for excitation, it uses maintenance-free LED lamp of long service life.

● Advanced thermoelectric refrigeration technology ensures fast and steady heating and refrigerating of ultra-fast heat cycle system.

●   SOAK Low Temperature Conservation function

●   Features the following functions: Absolute Quantification, Relative Quantification, SNP Analysis, Melting Curve, Gradient

●   Also variable about socket alternatives: RS232C Adapter or optionally USB adapter and Blue Tooth adapters are available.

DNA qPCR Probe Master Mix Kit

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Product Description

Gentaur DNA qPCR Probe Master Mix is a ready-to-use, master mix that contains all the reagents (except primers, probes and templates) needed for running quantitative, real-time DNA detection assays. Gentaur DNA qPCR Probe Master Mix is used for real-time detection and quantitation of DNA.

Intended Use

Gentaur DNA qPCR Probe Master Mix is used for real-time detection and quantitation of DNA. It can be also utilized for several purposes including the analyzing of gene expression, microbial detection and the determination of viral load.

Catalog No

Test No

09030010

10 Test

09030100

100 Test

 

Viral RNA Isolation Kit

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Serum and plasma samples of viral RNA to achieve high purity and molecular medicine is of great importance in research disciplines. Of serum and plasma samples containing viral agents RT Viral RNA isolation kit enables high purity, fast and simple method to obtain viral RNA. Enzymatic studies can be completed in 20-25 minutes without a transaction. The viral RNA was designed by silica-based spin columns and optimized thanks to the content of lysis and washing solutions by treatment of viral RNA with high efficiency and purity can be obtained.

Downstream Applications

Purified total RNA, RT-PCR, Real-Time RT-PCR in molecular techniques such as Northern blots can easily

Format

Spin column

Sample

150 μl Serum or plasma

Yield

Variable according to viral load

Speed

About 25 minutes

 

Catalog No

Test Size

09006050

50 tests

09006100

100 tests

Viral Nucleic Acid Isolation Kit

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General Information

Gentaur Viral NA Isolation Kit can get highly purified RNA and DNA at same time in a single application from viruses in serum or plasma samples, offers a high degree of efficiency, and is practical and affordable for use in a diagnostic clinical laboratory.

Isolation of high-purity viral nucleic acid from serum and plasma samples is essential for clinical diagnosis and molecular research. Gentaur Viral Nucleic Acid Isolation Kit provides a rapid and simplemethod for isolation of highly pure viral nucleic acid from virus containing cell-free body fluids. Due to the simple procedure of the kit that requires only basic laboratory equipment, it is possible to isolate viral nucleic acids from various samples simultaneously.

Downstream Applications

The recovered viral nucleic acids can be used directly in downstream applications such as RT-PCR, qRT-PCR, qPCR, viral detection, viral load determination and viral genotyping.

Format

Spin column

Sample

500 μl Serum or plasma

Yield

Variable according to viral load

Purity

A 260 / A 280 ratio: 1, 80 – 1,90

Speed

About 25 minutes

 

Catalog No

Test Size

09029050

50 test

09029100

100 test

Viral DNA Isolation Kit

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Viral DNA Isolation Kit

Some applications of nucleic acid detection require very sensitive techniques while the efficiency, in terms of recovery rate of extraction and purification of nucleic acid, becomes crucial. For example, PCR has made possible the rapid laboratory diagnosis and immediate management of some viral infections. Viruses are often present in very low concentrations in biological fluids, such as serum or plasma.  Gentaur Viral DNA Isolation Kit enables isolation of high quality DNA from viruses in serum or plasma samples, offers a high degree of efficiency, and is practical as well as affordable for use in clinical diagnostic laboratories.

Downstream Applications

The purified viral DNA is suitable for use in downstream applications such as PCR, Real-Time PCR, Southern Blot, Hybridization studies, Genotyping or Sequencing.

Format

Spin column

Sample

Serum or plasma

Yield

Variable according to viral load

Purity

A 260 / A 280 ratio: 1, 80 – 1,90

Speed

About 25 minutes

Catalog No

Test Size

09006050

50 test

09006100

100 test

 

Plasmid DNA Isolation Kit

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General Information

Gentaur Plasmid DNA Isolation Kit provides a rapid and easy method for purifying highly pure plasmid DNA from bacterial cultures by use of specially designed plasmid DNA specific spin columns and optimized composition of buffers.

Downstream Applications

The purified plasmid DNA is suitable for use in downstream applications such as PCR, Real-Time PCR, Cloning, Transformation, Restriction Enzyme Digestion, Ligation, Hybridization studies, Genotyping, or Sequencing.

Format

Spin column

Sample

Bacterial culture in liquid media

Yield

Up to 20 µg plasmid DNA

Purity

A 260 / A 280 ratio: 1,80 – 1,90

Speed

About 20 minutes

 

Catalog No

Test Size

09007050

50 tests

09007100

100 tests

 

Genomic DNA Isolation Kit from Yeast

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General Information

Gentaur Genomic DNA Isolation Kit from Yeast is optimized for isolation of highly purified DNA from a variety of yeast species such as Candida, Saccharomyces, Pichia, and Schizosaccharomyces. In contrast to DNA isolation protocols from human cells or viruses, protocols for extraction of genomic DNA from yeast cells are either very time-consuming or results in poor release of yeast DNA. The rather tough cell wall of the yeast cell must be removed prior to the DNA isolation process by digestion with lyticase or zymolase.  From the resulting spheroplasts, it will be very easy to lyse the yeast cells and quickly release the DNA in the cell into the environment.

Downstream Applications

The purified genomic DNA is suitable for use in downstream applications such as PCR, Real-Time PCR, Southern Blot, Restriction Enzyme Digestion, Cloning, Hybridization studies, Genotyping, or Sequencing.

Sistem

Yeast culture in liquid media or agar plates

Örnek

Variable according to the type and number of the yeast cells

Miktar

A 260 / A 280 ratio: 1, 80 – 1,90

Saflık

Yeast culture in liquid media or agar plates

Süre

About 2.5 hours

 

Catalog No

Test Size

09002050

50 tests

09002100

100 tests

Genomic DNA Isolation Kit From Tissue

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General Information

Gentaur Genomic DNA Isolation Kit from Tissue provides a rapid and easy method for isolation of pure genomic DNA from tissues and paraffin-embedded tissues. In contrast to the laborious and time-consuming traditional methods employing hazardous organic solvents, the kit follows a simple, efficient and fast methodology. Containing genomic DNA specific spin columns and solutions with specially optimized compositions, high quality genomic DNA with high yield is obtained at the end of the procedure. The kit is efficient in extracting genomic DNA from all kinds of solid tissues.

Downstream Applications

The purified genomic DNA is suitable for use in downstream applications such as PCR, Real-Time PCR, Southern Blot, Restriction Enzyme Digestion, RFLP analysis, Hybridization studies, SNP analyses, Genotyping, or Sequencing.

Format

Spin column

Sample

Tissue or paraffin-embedded tissue

Yield

Variable according to the tissue source

Purity

A 260 / A 280 ratio: 1, 80 – 1,90

Speed

Total processing time depends on the tissue source. 

Catalog No

Test Size

09003050

50 tests

09003100

100 tests

Genomic DNA Isolation Kit from Blood

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General Information

Gentaur Genomic DNA Isolation Kit from Blood provides a fast and easy method for obtaining high purity chromosomal DNA from blood samples by using genomic DNA specific spin columns and solutions with optimized composition. In contrast to the laborious and time-consuming traditional methods employing hazardous organic solvents, the kit follows a simple, economical and fast methodology.

Downstream Applications

The purified genomic DNA is suitable for use in downstream applications such as PCR, Real-Time PCR, Southern Blot, Restriction Enzyme Digestion, RFLP analysis, Hybridization studies, SNP analyses, Genotyping, or Sequencing

Format

Spin column

Sample

Fresh blood or frozen blood preserved with citrate, heparin or EDTA

Yield

6 µg DNA from 200 µl blood sample

Purity

A 260 / A 280 ratio: 1,80 – 1,90

Speed

About 25 minutes

 

Catalog No

Test Size

09001050

50 tests

09001100

100 tests

Genomic DNA Isolation Kit from Bacteria

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General Information

Gentaur Gnomic DNA Isolation Kit from Bacteria enables isolation of highly purified DNA from a variety of bacteria species such as Gram-positive bacteria, Gram-negative bacteria, and mycobacteria. The kit is suitable for efficient DNA isolation from liquid cultures as well as from agar plates. By thesimple, efficient and fast methodology provided by the DNA specific spin columns and solutions with specially optimized compositions, high quality genomic DNA of high yield is obtained at the end of the procedure.

Downstream Applications

The purified genomic DNA is suitable for use in downstream applications such as PCR, Real-Time PCR, Cloning, Restriction Enzyme Digestion, Ligation, Hybridization studies, Genotyping, or Sequencing.

Format

Spin column

Sample

Bacterial culture in liquid media or on agar plates

Yield

Variable according to the type and concentration of bacterial cells

Purity

A 260/A 280 ratio: 1.80-1.90

Speed

1.5 hours for Gram-negative bacteria, 2 hours for Gram-positive bacteria, 3 hours for mycobacteria

 

Catalog No

Test Size

09005050

50 tests

09005100

100 tests